Recombinant Human IL-2 Protein, Fc Tag (MALS verified), 100µg

Background
Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.

Source
Recombinant Human IL-2 Protein, Fc Tag (IL2-H5269) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1). Predicted N-terminus: Ala 21

Molecular Characterization
This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 41.9 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Depletion of polyfunctional CD26highCD8+ T cells repertoire in chronic lymphocytic leukemia"
Bozorgmehr, Hnatiuk, Peters et al
Exp Hematol Oncol (2023) 12 (1), 13
(2) "The comparative analysis of selected interleukins and proinflammatory factors in CSF among de novo diagnosed patients with RRMS"
Morawiec, Techmański, Tracz et al
Clin Neurol Neurosurg (2022) 225, 107522
(3) "C-terminal citrullinated peptide alters antigen-specific APC:T cell interactions leading to breach of immune tolerance"
Malcolm, Nyirenda, Brown et al
J Autoimmun (2023) 135, 102994
Showing 1-3 of 87417 papers.