Recombinant Rat IL-2 R beta&IL-2 R gamma Heterodimer Protein, His Tag&Twin-Strep Tag (MALS verified), 100 µg

Background
Both Interleukin-2 receptor subunit beta and Interleukin-2 receptor subunit gamma are receptor for interleukin-2. Common subunit for the receptors for a variety of interleukins. Interacts with SHB upon interleukin stimulation. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Source
Recombinant Rat IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag (ILG-R5283) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Glu 239 (IL-2RB) & Ser 25 - Ala 262 (IL-2RG) (Accession # P26896-1(IL-2RB) & Q68FU6-1(IL-2RG)).
Predicted N-terminus: Ala 27 (IL-2RB) & Ser 25 (IL-2RG)

Molecular Characterization
Rat IL-2RB&IL-2RG Heterodimer Protein, His Tag&Twin-Strep Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 30.2 kDa (IL-2RB) and 34.7 kDa (IL-2RG). Subunit IL-2RB is fused with a polyhistidine tag at the C-terminus and subunit IL-2RG is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 40-50 kDa and 55-65 kDa respectively due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Immune-Enhancing Effect of Submerged Culture of Ceriporia lacerata Mycelia on Cyclophosphamide-Induced Immunosuppressed Mice and the Underlying Mechanisms in Macrophages"
Hwang, Lee, Pham et al
Int J Mol Sci (2022) 23 (2)
(2) "TGF-β1 potentiates Vγ9Vδ2 T cell adoptive immunotherapy of cancer"
Beatson, Parente-Pereira, Halim et al
Cell Rep Med (2021) 2 (12), 100473
(3) "Supplementation of Alhagi honey polysaccharides contributes to the improvement of the intestinal immunity regulating the structure of intestinal flora in mice"
Cai, Wusiman, Gu et al
Food Funct (2021) 12 (20), 9693-9707
Showing 1-3 of 272 papers.