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Recombinant Biotinylated Human IL-6, epitope tag free, ultra sensitivity (primary amine labeling), 25µg  

Recombinant Biotinylated Human IL-6, epitope tag free, ultra sensitivity (primary amine labeling), 25µg

MABSol® Recombinant Biotinylated Human IL-6, AA Val 30 - Met 212, expressed from human HEK293 cells, epitope tag free, ultra sensitivity (primary amine labeling)

Synonym
recombinant, biotinylated, human, IL6, HGF, Interleukin-6, BSF2, HSF, IFNB2

More details

IL6-H8218-25

Availability: within 7 days

348,00 €

Background
Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

Source
MABSol® Recombinant Biotinylated Human IL-6, epitope tag free, primary amine labeling (IL6-H8218) is expressed from human HEK293 cells. It contains AA Val 30 - Met 212 (Accession # NP_000591). It is the biotinylated form of ActiveMax® Human IL-6, Tag Free (IL6-H4218).
Predicted N-terminus: Val 30

Molecular Characterization
The product does NOT contain any epitope tags.
The protein has a calculated MW of 20.8 kDa. The protein migrates as 24-27 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

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(2) "Minocycline Pretreatment Prevents Blood-Brain Barrier Disruption in Septic Rats"
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(3) "Small secretory proteins of immune cells can modulate gynecological cancers"
Kumar, Vyas, Agnihotri et al
Semin Cancer Biol (2022)
Showing 1-3 of 163445 papers