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Recombinant Biotinylated Human IL-6, epitope tag free, ultra sensitivity (primary amine labeling), 25µg  

Recombinant Biotinylated Human IL-6, epitope tag free, ultra sensitivity (primary amine labeling), 25µg

MABSol® Recombinant Biotinylated Human IL-6, AA Val 30 - Met 212, expressed from human HEK293 cells, epitope tag free, ultra sensitivity (primary amine labeling)

recombinant, biotinylated, human, IL6, HGF, Interleukin-6, BSF2, HSF, IFNB2

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Availability: within 7 days

348,00 €

Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells, to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

MABSol® Recombinant Biotinylated Human IL-6, epitope tag free, primary amine labeling (IL6-H8218) is expressed from human HEK293 cells. It contains AA Val 30 - Met 212 (Accession # NP_000591). It is the biotinylated form of ActiveMax® Human IL-6, Tag Free (IL6-H4218).
Predicted N-terminus: Val 30

Molecular Characterization
The product does NOT contain any epitope tags.
The protein has a calculated MW of 20.8 kDa. The protein migrates as 24-27 kDa on a SDS-PAGE gel under reducing (R) condition due to different glycosylation.

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Less than 1.0 EU per μg by the LAL method.

>95% as determined by SDS-PAGE.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Please refer to product data sheet.

Clinical and Translational Updates

(1) "Apoptosis triggered by cytolethal distending toxin B subunit of Helicobacter hepaticus is aggravated by autophagy inhibition in mouse hepatocytes"
Qian, Cao, Wang et al
Biochem Biophys Res Commun (2022) 598, 40-46
(2) "Minocycline Pretreatment Prevents Blood-Brain Barrier Disruption in Septic Rats"
Yang, Cao, Wang et al
J Surg Res (2022) 273, 247-254
(3) "Small secretory proteins of immune cells can modulate gynecological cancers"
Kumar, Vyas, Agnihotri et al
Semin Cancer Biol (2022)
Showing 1-3 of 163445 papers