Recombinant Cynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, His tag, 100µg

Background
Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Source
Recombinant Cynomolgus / Rhesus macaque PD-L1, His Tag (PD1-C52H4) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # G7PSE7-1). In the region Phe 19 - Arg 238, the AA sequence of Cynomolgus and Rhesus macaque PD-L1 are homologus.
Predicted N-terminus: Phe 19

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.1 kDa. The protein migrates as 33-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Engineering tumor-specific gene nanomedicine to recruit and activate T cells for enhanced immunotherapy"
Wang, Zhou, Wang et al
Nat Commun (2023) 14 (1), 1993
(2) "Metformin inhibits neutrophil extracellular traps-promoted pancreatic carcinogenesis in obese mice"
Wang, Gao, Dai et al
Cancer Lett (2023)
(3) "Development of mouse models with restricted HLA-B*57:01 presentation for the study of flucloxacillin-driven T-cell activation and tolerance in liver injury"
Ananthula, Krishnavenisivakumar, Cardone et al
J Allergy Clin Immunol (2023)
Showing 1-3 of 24527 papers.

Citations

(1) "A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade"
Authors: Geuijen C, Tacken P, Wang LC, et al.
Journal: Nature communications 2021
Application: SPR
(2) "ANTIBODIES AGAINST PD-L1"
Authors: I Altintas, D Satijn, BE Van Den, D Verzijl
Journal: US20200239579A1 2020
Application: BLI
(3) "Novel anti-PD-1 antibodies"
Authors: X Qian, et al
Journal: US20190330348A1 2019
Application: ELISA