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Recombinant Cynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, Fc Tag, 100µg  

Recombinant Cynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, Fc Tag, 100µg

Recombinant Cynomolgus / Rhesus macaque PDL1 / B7-H1 / CD274 Protein, Phe 19 - Arg 238, produced in human 293 cells (HEK293), Fc Tag

Synonym
recombinant cynomolgus protein PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

More details

PD1-C5253-100

Availability: within 7 days

455,00 €

Background
Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Source
Recombinant Cynomolgus / Rhesus macaque PD-L1, Fc Tag (PD1-C5253) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # F6VEW6-1). In the region Phe 19 - Arg 238, the AA sequence of Cynomolgus and Rhesus macaque PD-L1 are homologus.
Predicted N-terminus: Phe 19

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 51.8 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "The RESP AI model accelerates the identification of tight-binding antibodies
Parkinson, Hard, Wang
Nat Commun (2023) 14 (1), 454
(2) "Associations of Tissue Tumor Mutational Burden and Mutational Status With Clinical Outcomes in KEYNOTE-042: Pembrolizumab Versus Chemotherapy For Advanced PD-L1 Positive NSCLC
Mok, Lopes, Cho et al
Ann Oncol (2023)
(3) "Exclusion of Patients with Autoimmune Disease in Lung Cancer Clinical Trials
Khan, Komiya
Asian Pac J Cancer Prev (2023) 24 (1), 331-336
Showing 1-3 of 23589 papers.

 

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