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Recombinant Mouse TIM-3 / HAVCR2 Protein, Fc Tag , 100µg  

Recombinant Mouse TIM-3 / HAVCR2 Protein, Fc Tag , 100µg

Recombinant Mouse TIM-3 / HAVCR2 Protein, Fc Tag (Mouse TIM-3, Fc Tag) Arg 20 - Arg 191 was produced in human 293 cells (HEK293).

Synonym: recombinant, mouse, protein, HAVCR2,TIM3,TIMD3,FLJ14428,KIM3

More details

TI3-M5252-100

Availability: within 7 days

348,00 €

Background
Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

Source
Reombinant Mouse TIM-3 / HAVCR2 Protein, Fc Tag (Mouse TIM-3, Fc Tag) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Arg 191 (Accession # AAL65156.1).
Predicted N-terminus: Arg 20

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 45.8 kDa. The protein migrates as 60-67 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse TIM-3, Fc Tag by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "TIM-3 in Leukemia; Immune Response and Beyond"
Rezaei, Tan, Zeng et al
Front Oncol (2021) 11, 753677
(2) "Expression Levels of Three Key Genes CCNB1, CDC20, and CENPF in HCC Are Associated With Antitumor Immunity"
Si, Huang, Jiang et al
Front Oncol (2021) 11, 738841
(3) "Increased expression of programmed cell death ligand 1 and galectin 9 in transplant recipients who achieved tolerance after immunosuppression withdrawal"
Hai Nam, Taura, Koyama et al
Liver Transpl (2021)
Showing 1-3 of 1859 papers.