Recombinant Cynomolgus B7-H3 / CD276 Protein, His Tag, 100µg

Background
B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.

Source
Recombinant Cynomolgus B7-H3, His Tag (B73-C52Ha) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Glu 465 (Accession # XP_015308534.1).
Predicted N-terminus: Leu 29

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 49.0 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "B7-H3 in Pediatric Tumors: Far beyond Neuroblastoma"
Bottino, Vitale, Dondero et al
Cancers (Basel) (2023) 15 (13)
(2) "Expression characteristic of 4Ig B7-H3 and 2Ig B7-H3 in acute myeloid leukemia"
Zhang, Zhang, Qian et al
Bioengineered (2021) 12 (2), 11987-12002
(3) "Preferential Induction of the T Cell Auxiliary Signaling Molecule B7-H3 on Synovial Monocytes in Rheumatoid Arthritis"
Yoon, Chung, Yoo et al
J Biol Chem (2016) 291 (8), 4048-57
Showing 1-3 of 10 papers.