Recombinant Biotinylated Human B7-H3 / CD276 Protein, Avitag™, His Tag, 25 µg

Background
B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.

Source
Recombinant Biotinylated Human B7-H3, Avitag,His Tag (B73-H82E6) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Pro 245 (Accession # Q5ZPR3-2).
Predicted N-terminus: Leu 29

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 26.5 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Alternative immune checkpoints in immunoregulatory profile of cancer stem cells"
Mortezaee, Majidpoor
Heliyon (2023) 9 (12), e23171
(2) "B7-H3 promotes angiogenesis in rheumatoid arthritis"
Yang, Xiong, Sun et al
Mol Immunol (2023) 165, 19-27
(3) "A novel Fc-enhanced humanized monoclonal antibody targeting B7-H3 suppresses the growth of ESCC"
Wu, Liu, Yuan et al
Oncoimmunology (2023) 12 (1), 2282250
Showing 1-3 of 874 papers.