Recombinant Human Galectin-9 / LGALS9 Protein, His Tag, 50 µg

Background
Galectin-9 (LGALS9) is also known as tumor antigen HOM-HD-21 and ecalectin. Galectins are a family of proteins defined by their binding specificity for β-galactoside sugars, such as N-acetyllactosamine (Galβ1-3GlcNAc or Galβ1-4GlcNAc), which can be bound to proteins by either N-linked or O-linked glycosylation. As for Galectin-9, it has high affinity for the Forssman pentasaccharide, and it is also the ligand for HAVCR2/TIM3. Forthermore, Galectin-9 stimulates bactericidal activity in infected macrophages by causing macrophage activation and IL1B secretion which restricts intracellular bacterial growth.

Source
Recombinant Human Galectin-9, His Tag (LG9-H5244) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Thr 323 (Accession # O00182-2).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 36.6 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Evaluation of the Relationship Between miRNA-22-3p and Gal-9 Levels in Glioblastoma"
Barut, Akdeniz
In Vivo (2023) 37 (6), 2577-2584
(2) The glucose transporter 2 regulates CD8+ T cell function via environment sensing"
Fu, Vuononvirta, Fanti et al
Nat Metab (2023)
(3) "Immunological profiling in long COVID: overall low grade inflammation and T-lymphocyte senescence and increased monocyte activation correlating with increasing fatigue severity"
Berentschot, Drexhage, Aynekulu Mersha et al
Front Immunol (2023) 14, 1254899
Showing 1-3 of 957 papers.

Citations

(1) "Activated Galectin-9/Tim3 promotes Treg and suppresses Th1 effector function in chronic lymphocytic leukemia"
Authors: Pang N, Alimu X, Chen R, et al.
Journal: FASEB journal 2021
Application: ELISA