Recombinant, human CSF1R protein, kinase domain, 10 µg

Background: CSF1R is a tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells. CSF1-R also known as M-CSF, is a critical regulator of the development, morphology, survival, and functions of tissue macrophages and osteoclasts and plays an important role in innate immunity and in inflammatory processes. It is a transmembrane glycoprotein composed of an Ig-like extracellular ligand-binding domain, a hydrophobic transmembrane domain, and an intracellular C-terminal tyrosine kinase domain that includes eight tyrosine residues, known to be phosphorylated in the activated receptor. Upon activation by lingand binding intracellular autophosphorylated tyrosines behave as docking sites for a variety of proteins that include several Src family kinases, the p85 subunit of PI3K, phospholipase Cγ2, suppressor of cytokine signaling-1, Grb2, and c-Cbl.

Human colony stimulating factor 1 receptor, C-terminal fragment, amino acids K543-C972 (as in NCBI/Protein entry NP_005202.2), recombinant and active enzyme, C-terminal fragment, amino acids K543-C972, N-terminal GST-HIS6 fusion protein with a Thrombin cleavage site, expressed in Sf9 insect cells

Theoretical MW: 77.733 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: GST-Affinity Chromatography
Storage buffer: 50 mM HEPES pH 7.5,100 mM NaCl, 5 mM DTT, 20% glycerol
Protein concentration: 0.117 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 50,000 pmol/mg x min

Entrez Gene ID: 1436
UniProtKB: P07333

Ordering information: shipped on dry ice

Product specific literature:

Bourette R.P., Mouchiroud G., Ouazana R., Morle F., Godet J., Blanchet J.P. (1993) "Expression of human colony-stimulating factor-1 (CSF-1) receptor in murine pluripotent hematopoietic NFS-60 cells induces long-term proliferation in response to CSF-1 without loss of erythroid differentiation potential." Blood 81:2511-2520

Baran C.P., Tridandapani S., Helgason C.D., Humphries R.K., Krystal G., Marsh C.B.(2003) "The inositol 5'-phosphatase SHIP-1 and the Src kinase Lyn negatively regulate macrophage colony-stimulating factor-induced Akt activity."J. Biol. Chem. 278:38628-38636

Droin N, Solary E. (2010)"CSF1R, CSF-1, and IL-34, a "menage a trois" conserved across vertebrates."J Leukoc Biol. 87(5):745-7.