Recombinant human Interleukin-2-inducible T-cell kinase (ITK), protein kinase, 10 µg

Background: Interleukin-2-inducible T-cell kinase (Itk) is a member of the Tec family of nonreceptor protein tyrosine kinases expressed primarily in hematopoietic cells and serve as important mediators of antigen receptor signaling in lymphocytes. Itk is recruited to the membrane via its PH domain in response to TCR activation and phosphorylates PLCγ, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty.

Human ITK (IL2-inducible T-cell kinase1), full length, amino acids M1-L630 (as in NCBI/Protein entry NP_005537.3), recombinant and active enzyme, N-terminally fused to GST-HIS6-Thrombin cleavage site

Theoretical MW: 101.727 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using GSH-agarose
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.073 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC membrane
Specific activity: 11,000 pmol/mg x min

Entrez Gene ID: 3702
UniProtKB: Q08881

Ordering information: shipped on dry ice

Product specific literature

Andreotti AH1, Schwartzberg PL, Joseph RE, Berg LJ. (2010)"T-cell signaling regulated by the Tec family kinase, Itk."Cold Spring Harb Perspect Biol. ;2(7):a002287. doi: 10.1101

Horwood NJ, Urbaniak AM, Danks L. (2012) "Tec family kinases in inflammation and disease."Int Rev Immunol. 2012 Apr;31(2):87-103.

Hussain A, Yu L, Faryal R, Mohammad DK, Mohamed AJ, Smith CI. (2011) "TEC family kinases in health and disease--loss-of-function of BTK and ITK and the gain-of-function fusions ITK-SYK and BTK-SYK."FEBS J. 278(12):2001-1