Recombinant Deoxycytidine kinase kinase (dCK), 50 µg

Deoxycytidine kinase (dCK) is a nucleoside kinase which is involved in the deoxynucleoside salvage-pathway and thus responsible for the conversion of deoxycytidine (dC), deoxyadenosine (dA) and deoxyguanosine (dG) into their monophosphate forms. By this means cells are supplied with deoxyribonucleotides for DNA replication and repair synthesis. In addition to its natural substrates dCK is also able to activate numerous nucleoside analogues used in cancer and antiviral chemotherapy. The enzyme is higly expressed in lymphoid tissues and beside there is an increase dCK activity in solid tumours.

dCK is recombinantly expressed in E. coli, fused to GST and purified using affinity chromatography MW. 56 kDa

Protein concentration:1.9 mg/ml
Purity:  > 90% by SDS PAGE
Specific activity: 0.5 U*/mg
Buffer: Tris-HCl, pH 8,0, 0.1% Triton X-100, 10 % glycerol

* 1 Unit is defined as 1 µmol phosphate  transferred to dC per min

Entrez Gene ID: 1633
UniProtKB:  P27707

Ordering information: shipped on dry ice

Product specific literature references:

Sabini E, Hazra S, Ort S, Konrad M, Lavie A. (2008) " Structural basis for substrate promiscuity of dCK." J Mol Biol. 378(3):607-21

Arnor ES, Eriksson S. (1995) "Mammalian deoxyribonucleoside kinases." Pharmacol Ther. 67(2):155-86

Munch-Petersen B, Tyrsted G, Cloos L (2004) "Activation of deoxycytidine kinase by protein kinase inhibitors and okadaic acid in leukemic cells " Biochem Pharmacol. 68(1):95-103