Recombinant human S100A6 Protein, Tag free, 100µg

Background
S100 calcium binding protein A6 (S100-A6), also kown as 2A9, 5B10, CABP, CACY and PRA, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100A6 may function in stimulation of Ca2+ -dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma. S100A6 has been shown to interact with S100B[1-2]and SUGT1.[3]

Source
Recombinant Human S100A6 Protein, Tag Free(S16-H5111) is expressed from E. coli cells. It contains AA Met 1 - Gly 90 (Accession # AAH01431).
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries no "tag"
The protein has a calculated MW of 10.2 kDa. The protein migrates as 10.2 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Multi-omics characteristics of tumor-associated macrophages in the tumor microenvironment of gastric cancer and their exploration of immunotherapy potential"
Cao, Liu, Cheng et al
Sci Rep (2023) 13 (1), 18265
(2) "Interaction of S100A6 Protein with the Four-Helical Cytokines"
Kazakov, Deryusheva, Rastrygina et al
Biomolecules (2023) 13 (9)
(3) "S100A6 is a potential diagnostic and prognostic biomarker for human glioma"
Hong, Zhang, Xiao et al
Oncol Lett (2023) 26 (4), 458
Showing 1-3 of 631 papers.