Recombinant human S100A8 Protein, Tag free, 100µg

Background
S100 calcium binding protein A8 (S100-A8), also kown as Cystic fibrosis antigen, Migration inhibitory factor-related protein 8 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF and P8, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from the neural crest, chondrocytes, macrophages, dendritic cells, etc. S100A8 plays a role in various functions of myeloid cells by forming a heterocomplex with S100A9. S100A8 and S100A9 are known to be overexpressed in certain species of carcinomas. S100A8 may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.[1] S100A8 plays an important role in differentiation of thyroid carcinoma possibly by forming a complex with S100A9. S100A8 and S100A9 may also play a key role in inflammation-associated cancer. [2]

Source
Recombinant Human S100A8 Protein (rhS100A8), Tag Free (S18-H5112) is expressed from E.coli cells. It contains AA Met 1 - Glu 93 (Accession # AAH05928).
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries no "tag".
The protein has a calculated MW of 10.8 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
N/A

Clinical and Translational Updates

(1) "E-selectin-mediated rapid NLRP3 inflammasome activation regulates S100A8/S100A9 release from neutrophils via transient gasdermin D pore formation"
Pruenster, Immler, Roth et al
Nat Immunol (2023)
(2) "Extracellular proteins as potential biomarkers in Sepsis-related cerebral injury"
Dong, Wang, Hu et al
Front Immunol (2023) 14, 1128476
(3) "Identification of Genes and Key Pathways Associated with the Pathophysiology of Lung Cancer and Atrial Fibrillation"
Yang, Chen, Yu et al
Altern Ther Health Med (2023)
Showing 1-3 of 2417 papers.