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Recombinant human CXADR / CAR Protein, His Tag, 100µg  

Recombinant human CXADR / CAR Protein, His Tag, 100µg

Recombinant Human CXADR / CAR Protein, Leu 20 - Gly 237, produced in human 293 cells (HEK293), His Tag

Synonym
Recombinant, human protein, CXADR, CAR, CAR4/6, HCAR

More details

CXR-H5222-100

Availability: within 7 days

300,00 €

Background
Coxsackie virus and adenovirus receptor (CXADR) also known as CAR, is a type I transmembrane glycoprotein for group B coxsackie viruses and subgroup C adenoviruses, and belongs to the CTX family of the Ig superfamily. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins such as: fibronectin, agrin, laminin-1 and tenascin-R. Human CXADR protein contains a signal sequence, a extracellular domain (ECD) with a V­ type (D1) and a C2­ type (D2) Ig­like domain, a  transmembrane segment and a intracellular domain. D1 is thought to be responsible for homodimer formation in trans within tight junctions, and is necessary and sufficient for adenovirus binding. Variants of CXADR are attached to the cell membrane by a GPI- anchor.[1-4]

Source
Recombinant Human CXADR / CAR Protein (rhCXADR) Leu 20 - Gly 237 (Accession # NP_001329.1) was produced in human 293 cells (HEK293).

Molecular Characterization
rhCXADR, fused with a C-terminal 6×His tag, has a calculated MW of 24.9 kDa expressed. The predicted N-terminus is Leu 20. Protein migrates as 35 kDa in reduced SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCXADR by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
N/A

References

(1)Bergelson JM, et al., 1997, Science 275 (5304): 1320–3.
(2)Tomko RP, et al., 1997, Proc Natl Acad Sci U S A 94 (7): 3352–6.
(3)Patzke C, et al., 2010, Journal of Neuroscience 30 (8): 2897–2910.
(4)Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-80.