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Recombinant Human C1q R1 / CD93 Protein, His Tag, 50 µg  

Recombinant Human C1q R1 / CD93 Protein, His Tag, 50 µg

Recombinant Human C1q R1 / CD93 Protein, Thr 22 - Lys 580, expressed from human 293 cells (HEK293), His Tag (MALS verified)

Synonym: Recombinant, human, protein, CD93, CDw93, C1QR1, MXRA4, C1qR, C1qR(P), C1qRP, ECSM3

More details

C11-H5228-50

Availability: within 7 days

377,00 €

Background
CD antigen CD93 is also known as Complement component C1q receptor (C1QR1). which belongs to the Group XIV C-Type lectin family, a group containing two other members, endosialin (CD248) and thrombomodulin. CD93 contains one C-type lectin domain and five EGF-like domains. CD93 is highly expressed in endothelial cells, platelets, cells of myeloid origin, such as monocytes and neutrophils. CD93 is receptor for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). CD93 may mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens and may play a role in intercellular adhesion.

Source
Recombinant Human C1q R1, His Tag (C11-H5228) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Lys 580 (Accession # AAH28075).
Predicted N-terminus: Thr 22

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 59.2 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Streptococcus pneumoniae interactions with the complement system"
Gil, Noursadeghi, Brown
Front Cell Infect Microbiol (2022) 12, 929483
(2) "Dominant role of splenic marginal zone lipid rafts in the classical complement pathway against S. pneumoniae"
Yang, Park, Choi et al
Cell Death Discov (2019) 5, 133
(3) "How antibodies use complement to regulate antibody responses"
Sörman, Zhang, Ding et al
Mol Immunol (2014) 61 (2), 79-88
Showing 1-3 of 14 papers.