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Recombinant Mouse Dkk-3 Protein, His Tag, 50 µg  

Recombinant Mouse Dkk-3 Protein, His Tag, 50 µg

Recombinant Mouse DKK-3 /DKK3 Protein (Pro 23 - Ile 349), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant mouse protein, DKK3,REIC,RIG

More details

DK3-M5226-50

Availability: within 7 days

348,00 €

Background
Members of the dickkopf-related protein family (DKK-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. And DKK3 has been proposed as tumour suppressor gene and a marker for tumour blood vessels. DKK3 is the only DKK family member abundantly expressed in normal lung, but silenced by promoter hypermethylation in a large fraction of lung cancer cell lines and lung tumors. Downregulation of DKK3 was correlated with tumor progression and expression of nuclear beta-catenin in lung tumors. Ectopic expression of DKK3 in lung cancer cells with DKK3 hypermethylation induced apoptosis and inhibited TCF-4 activity as well as nuclear accumulation of beta-catenin and expression of TCF-4 targets c-Myc and cyclin D1. DKK3 modulates FGF and Activin/Nodal signaling to regulate mesoderm induction during early Xenopus development, was reported.

Source
Recombinant Mouse DKK-3 /DKK3 Protein (Mouse DKK3) Pro 23 - Ile 349 (Accession # NP_056629.1) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
Mouse DKK3 is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 37.1 kDa. The predicted N-terminus is Pro 23. DTT-reduced Protein migrates as 55-66 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Mouse DKK3 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

References

(1) Medinger M, Tzankov A,et al., 2011, Thromb Haemost. 105(1):72-80.
(2) Yue W, Sun Q, et al., 2008, Carcinogenesis. 29(1):84-92..
(3) Pinho S, Niehrs C.et al., 2007, Differentiation. 75(10):957-67.