Recombinant Mouse Properdin Protein, His Tag, 100 µg

Background
Properdin is also known as Complement factor P, CFP, PFC, a positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Properdin is produced by many different leukocyte subsets and circulates as cyclic oligomers of monomeric subunits. Properdin on platelet/granulocyte aggregates(PGA) formation are tightly regulated by Factor H, properdin enhances PGA formation via increased production of C5a, and that inhibition of properdin function has therapeutic potential to limit thromboinflammation in diseases characterized by increased PGA formation.

Source
Recombinant Mouse Properdin, His Tag (PRN-M52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Pro 464 (Accession # P11680-1).
Predicted N-terminus: Ser 23

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 49.8 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "The role of gamma globulin, complement component 1q, factor B, properdin, body temperature, C-reactive protein and serum amyloid alpha to the activity and the function of the human complement system and its pathways"
Atosuo, Karhuvaara, Suominen et al
J Immunol Methods (2024)
(2) "Paired plasma lipidomics and proteomics analysis in the conversion from mild cognitive impairment to Alzheimer's disease"
Gómez-Pascual, Naccache, Xu et al
Comput Biol Med (2024) 176, 108588
(3) "Severe Meningococcal Meningitis Revealing a Novel Form of Properdin Deficiency in a Previously Healthy 13-year-old Child"
Bevacqua, Bastard, Pinhas et al
Pediatr Infect Dis J (2024)
Showing 1-3 of 2433 papers.