Recombinant Mouse Properdin Protein, His Tag, 100 µg

Background
Properdin is also known as Complement factor P, CFP, PFC, a positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Properdin is produced by many different leukocyte subsets and circulates as cyclic oligomers of monomeric subunits. Properdin on platelet/granulocyte aggregates(PGA) formation are tightly regulated by Factor H, properdin enhances PGA formation via increased production of C5a, and that inhibition of properdin function has therapeutic potential to limit thromboinflammation in diseases characterized by increased PGA formation.

Source
Recombinant Mouse Properdin, His Tag (PRN-M52H5) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Pro 464 (Accession # P11680-1).
Predicted N-terminus: Ser 23

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 49.8 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "What lies in-between: C3 glomerulopathy with non-hemolytic renal microangiopathy and an ultra-rare C3 variant"
Jandal, Zhong, Gopal et al
Am J Med Sci (2022)
(2) "Human coagulation factor X and CD5 antigen-like are potential new members of the zonulin family proteins"
Konno, Martinez, Ji et al
Biochem Biophys Res Commun (2023) 638, 127-133
(3) "MS-proteomics provides insight into the host responses towards alginate microspheres"
Coron, Fonseca, Sharma et al
Mater Today Bio (2022) 17, 100490
Showing 1-3 of 2402 papers.