anti-ERK1/2 antibody, 100 µg

ERK1 and ERK2, also known MAPK3 and MAPK1, are MAP kinases which are activated by MAPK kinase (MEK or MKK). The antibody was raised against a synthetic peptide mapping at the N-terminal of the human MAPK1+3.  Specific for ERK1 and ERK2 (human,  mouse, rat, rabbit), no cross reactivity with other proteins. Immunogen affinity purified antibody. Reconstitution: 1.2% sodium acetate or neutral PBS. 

Applications:

• Western Blot Analysis: use at 0.5-1.0 µg/ml
• Immunohistochemistry: use at 1-2 µg/ml

Product specific literature references:

Ahn, N.G. "The MAP kinase cascade. Discovery of a new signal transduction pathway," Mol.
Cell Biochem. 127 - 128 (1993) 201 - 209.

Adams, J.P. and Sweatt, J.D. "Molecular psychology: Roles for the ERK MAP kinase
cascade in memory," Annu. Rev. Pharmacol. Toxicol. 42 (2002) 135 - 163.

Atkins, C.M., Selcher, J.C., Petraitis, J.J., Trzaskos, J.M. and Sweatt, J.D. "The MAPK
cascade is required for mammalian associative learning," Nat. Neurosci. 1 (1998) 602 - 609.

Charest, D.L., Mordret, G., Harder, K.W., Jirik, F. and Pelech, S.L. "Molecular cloning,
expression, and characterization of the human mitogen-activated protein kinase p44erk1,"
Mol. Cell Biol. 13 (1993) 4679 - 4690.

Johnson, G.L. and Lapadat, R. "Mitogen-activated protein kinase pathways mediated by
ERK, JNK, and p38 protein kinases," Science, 298 (2002)1911-1912.