Recombinant HCoV-229E Spike protein, His Tag, 50µg

Background
Human coronavirus 229E (HCoV-229E) is one of seven known coronaviruses to infect humans. Likely originated from bats, it is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by binding to the APN receptor. Along with Human coronavirus OC43 (a member of the Betacoronavirus genus), it is one of the viruses responsible for the common cold. The spike protein of HCoV-229E is a trimer with subunits S1 and S2 responsible for host receptor binding and fusion of the viral and host cell membranes, respectively.

Source
Recombinant HCoV-229E Spike Protein, His Tag (SPN-H52H3) is expressed from human 293 cells (HEK293). It contains AA Cys 16 - Pro 1115 (Accession # P15423-1 (TI 871-872 PP)).The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the N-terminus. Proline substitutions TI 871-872 PP are introduced to stabilize the trimeric prefusion state of the spike protein.
Predicted N-terminus: Cys 16

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 125.7 kDa. The protein migrates as 150-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

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