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Recombinant Cynomolgus / Rhesus macaque FcRn/FCGRT & B2M Heterodimer Protein, 50 µg  

Recombinant Cynomolgus / Rhesus macaque FcRn/FCGRT & B2M Heterodimer Protein, 50 µg

Recombinant Cynomolgus FCGRT & B2M heterodimer protein (Ala 24 - Ser 297, FCGRT, Q8SPV9) and & Ile 21 - Met 119 (B2M, Q6V7J5) was produced in human 293 cells (HEK293), Strep II-tag, His-tag, (MALS & BLI verified)

Synonyms: recombinant, cynomolgus, protein FcRN & B2M, FCGRT & B2M

More details

FCM-C5284-50

420,00 €

Background
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Source
Recombinant Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (FCM-C5284), is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # Q8SPV9-1(FCGRT) & Q8SPW0-1(B2M)). In the region Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M), the AA sequence of Cynomolgus and Rhesus macaque FcRn (FCGRT & B2M) are homologus.
Predicted N-terminus: Ala 24 (FCGRT) & Ile 21 (B2M)

Molecular Characterization
Cynomolgus / Rhesus macaque FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 36 kDa (FCGRT) and 14 kDa (B2M) respectively due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

References

(1) "Polymorphisms of neonatal Fc receptor in cynomolgus and rhesus macaques"
Uno, Utoh, Iwasaki
Drug Metab Pharmacokinet (2014) 29 (5), 427-30
(2) "Humanized FcRn mouse models for evaluating pharmacokinetics of human IgG antibodies"
Proetzel, Roopenian
Methods (2014) 65 (1), 148-53
Showing 1-2 of 2 papers.
Please refer to product data sheet.

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