Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Biotinylated Human FcRn / FCGRT & B2M, ultra sensitivity (primary amine labeling), 25 µg  

Recombinant Biotinylated Human FcRn / FCGRT & B2M, ultra sensitivity (primary amine labeling), 25 µg

Biotinylated Human FcRn / FCGRT&B2M Heterodimer Protein, AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M), His Tag&Strep II Tag, expressed from human 293 cells (HEK293), ultra sensitivity (primary amine labeling) (SPR verified)

Synonyms: recombinant human, protein FcRN & B2M,FCGRT & B2M

More details

FCM-H8286-25

420,00 €

Background:
FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.

Source
Recombinant Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling (FCM-H8286) is expressed from human 293 cells (HEK293). It contains AA Ala 24 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M) (Accession # P55899-1 (FCGRT) & P61769-1 (B2M)). It is the biotinylated form of Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR & BLI verified) (FCM-H5286).
Predicted N-terminus: Ala 24(FCGRT) & Ile 21(B2M)

Molecular Characterization
Biotinylated Human FCGRT&B2M, His Tag&Strep II Tag, primary amine labeling, produced by co-expression of FCGRT and B2M, has a calculated MW of 31.2 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 33 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.

Biotinylation
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

The biotin to protein ratio is 2.5-4.5 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22µm filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Contact us for customized product form or formulation.

Reconstitution
Reconstitute at 100 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Please refer to product data sheet.

Bioactivity
Please refer to product data sheet.

BIAFFIN SERVICES:

Use BIAFFINS FcRn BINDING ASSAYS for detailed kinetic analysis of your antibodies using SPR-based assays.

Figure: Real-time kinetic analysis of antibody binding to FcRn using surface plasmon resonance.

Please contact our application specialists to obtain more information and an individual quote tailored to your specific needs.

References

(1) Goebl NA., et al., 2008, Mol. Biol. Cell 19 (12): 5490–505.
(2) Lee TY., et al., 2008, Clin. Cancer Res. 14 (5): 1487–93.
(3) Güssow D., et al., 1987, J. Immunol. 139 (9): 3132–8.
(4) Gorevic P.D., et al., 1986, Proc. Natl. Acad. Sci. U.S.A. 83:7908-7912.

The following products could also be interesting for you: