Recombinant Human MICA Protein, His Tag (MALS verified), 100 µg

Background
MHC class I polypeptide-related sequence A (MICA) belongs to the MHC class I family and MIC subfamily. MICA contains one Ig-like C1-type (immunoglobulin-like) domain. Unlike classical MHC class I molecules, MICA does not form a heterodimer with beta-2-microglobulin. MICA acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. MICA is ligand for the KLRK1/NKG2D receptor. MICA bind to KLRK1 leads to cell lysis.

Source
Recombinant Human MICA, His Tag (MIA-H5221) is expressed from human 293 cells (HEK293). It contains AA Glu 24 - Gln 308 (Accession # AAH16929.1).
Predicted N-terminus: Glu 24

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33.8 kDa. The protein migrates as 45-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1)  "KLF4-mediated upregulation of the NKG2D ligand MICA in acute myeloid leukemia: a novel therapeutic target identified by enChIP"
Alkhayer, Ponath, Frech et al
Cell Commun Signal (2023) 21 (1), 94
(2) "Vertically Aligned One-Dimensional Crystal-Structured Sb2Se3 for High-Efficiency Flexible Solar Cells via Regulating Selenization Kinetics"
Wen, Lu, Yang et al
ACS Appl Mater Interfaces (2023)
(3)  "Implementing microwave impedance microscopy in a dilution refrigerator"
Jiang, Chong, Zhang et al
Rev Sci Instrum (2023) 94 (5)
Showing 1-3 of 6386 papers.

Citations

(1) "Shedding of CD16 disassembles the NK cell immune synapse and boosts serial engagement of target cells"
Authors: Srpan K, et al.
Journal: J Cell Biol 2018
Application: Stimulate NK cells