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Recombinant Biotinylated Human Siglec-3 / CD33 Protein, Avi Tag (Avitag™), 25µg  

Recombinant Biotinylated Human Siglec-3 / CD33 Protein, Avi Tag (Avitag™), 25µg

MABSol® Recombinant Biotinylated Human Siglec-3 / CD33, His Tag, expressed from human 293 cells (HEK293), Avi tag (Avitag™), His Tag

Synonym
recombinant, human, biotinylated, protein, CD33,SIGLEC3,gp67

More details

CD3-H82E7-25

Availability: within 7 days

420,00 €

Background
Myeloid cell surface antigen CD33 also known as SIGLEC3, Siglecs (sialic acid binding Ig­like lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-­terminal Ig-­like V­-type domain, one Ig­-like C2-­type domains, a transmembrane region and a cytoplasmic tail. CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells. In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.

Source
MABSol®Recombinant Biotinylated Human Siglec-3 / CD33, His Tag (CD3-H82E7) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
Predicted N-terminus: Asp 18

Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 29.4 kDa. The protein migrates as 45-55 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by reduced SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Reconstitute at 100 μg/mL in sterile deionized water.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Bioactivity
Immobilized anti human CD33 MAb, Mouse IgG1 (Clone # 6C5/2) at 2μg/mL (100 μL/well) can bind Biotinylated Human Siglec-3, His Tag (Catalog # CD3-H82E7) with a linear range of 0.078-2.5 ng/mL.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
•4-8°C for 12 months in lyophilized state;
•-70°C for 3 months under sterile conditions after reconstitution.

References

(1) Garnache-Ottou F., et al., 2005, Blood 105 (3): 1256–64.
(2) Hernández-Caselles T, et al., 2006, J. Leukoc. Biol. 79 (1): 46–58.
(3) Walter RB, et al., 2007, Blood 109 (10): 4168–70.
(4) Ulyanova, T. et al., 1999, Eur. J. Immunol. 29:3440.
(5) Crocker, P.R. and A. Varki, 2001, Immunology 103:137.

Limited Use License
The Biotin AviTag technology is covered by U.S. Pat. No: 5,874,239 and includes any and all materials, methods, kits and related derivatives claimed by this patent.
The purchase of the Acrosbiosystems’s Avitag™ proteins confers to the purchaser the limited right to use the Avitag™ technology for non-commercial, or research use, or for purposes of evaluating the Avitag™ technology.