Recombinant Human Glutaredoxin 1 / GLRX1 Protein, His Tag, 100 µg

Background
Glutaredoxin-1 (GRX1) is also known as Thioltransferase-1 (TTase-1), GLRX, GRX, GLRX1, which belongs to the glutaredoxin family. GRX1 / GLRX contains one glutaredoxin domain, which exists in either a reduced or an oxidized form. GRX1 / GLRX has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase and functions as electron carriers in the glutathione-dependent synthesis of deoxyribonucleotides by the enzymeribonucleotide reductase.

Source
Recombinant Human Glutaredoxin 1, His Tag (GLX-H5149) is expressed from E.coli cells. It contains AA Met 1 - Gln 106 (Accession # NP_001112362).
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 12.7 kDa. The protein migrates as 12 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with 1 mM DTT. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Fmp40 ampylase regulates cell survival upon oxidative stress by controlling Prx1 and Trx3 oxidation"
Masanta, Wiesyk, Panja et al
Redox Biol (2024) 73, 103201
(2) "An evolutionary view of CC-type glutaredoxins functions in land plants development and adaptation to environment"
El Baidouri, Reichheld, Belin
J Exp Bot (2024)
(3) "Requirements for the biogenesis of [2Fe-2S] proteins in the human and yeast cytosol"
Braymer, Stehling, Stümpfig et al
Proc Natl Acad Sci U S A (2024) 121 (21), e2400740121
Showing 1-3 of 1678 papers.