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Recombinant Human FKBP12 / FKBP1A Protein, His Tag, 100 µg  

Recombinant Human FKBP12 / FKBP1A Protein, His Tag, 100 µg

Recombinant Human FKBP12 / FKBP1A Protein, His Tag

Synonym
recombinant, FKBP1A, FKBP1, Calstabin-1, FKBP12, Rotamase, PKC12, PPIASE

More details

FKA-H5122-100

169,00 €

Background
Peptidyl-prolyl cis-trans isomerase FKBP1A, a member of the FKBP-type PPIase family and FKBP1 subfamily, is also known as FK506-binding protein 1A, immunophilin FKBP12 and 12 kDa FK506-binding protein, which contains one PPIase FKBP-type domain. FKBP1A is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 (tacrolimus) and rapamycin (sirolimus).FKBP1A interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. FKBP1A can also interact with multiple intracellular calcium release channels including the tetrameric skeletal muscle ryanodine receptor. Furthermore, FKBP1A can recruite SMAD7 to ACVR1B which prevents the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal.

Source
Recombinant Human FKBP12, His Tag (FKA-H5122) is expressed from E.coli cells. It contains AA Met 1 - Glu 108 (Accession # P62942-1).
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 12.8 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Calcineurin Inhibitor CN585 Exhibits Off-Target Effects in the Human Fungal Pathogen Aspergillus fumigatus"
Juvvadi, Bobay, Cole et al
J Fungi (Basel) (2022) 8 (12)
(2) "FK506-binding protein, FKBP12, promotes serine utilization and negatively regulates threonine deaminase in fission yeast"
Sasaki, Nishimura, Yashiroda et al
iScience (2022) 25 (12), 105659
(3) "Preventing Surgery-Induced NK Cell Dysfunction Using Anti-TGF-β Immunotherapeutics"
Market, Tennakoon, Scaffidi et al
Int J Mol Sci (2022) 23 (23)
Showing 1-3 of 1559 papers.