Recombinant HCoV-229E S1 protein, His Tag, 100µg

Background
Human coronavirus 229E (HCoV-229E) is one of seven known coronaviruses to infect humans. Likely originated from bats, it is an enveloped, positive-sense, single-stranded RNA virus which enters its host cell by binding to the APN receptor. Along with Human coronavirus OC43 (a member of the Betacoronavirus genus), it is one of the viruses responsible for the common cold. The spike protein of HCoV-229E is a trimer with subunits S1 and S2 responsible for host receptor binding and fusion of the viral and host cell membranes, respectively.

Source
Recombinant HCoV-229E S1 protein, His Tag (SIN-V52H4) is expressed from human 293 cells (HEK293). It contains AA Cys 16 - Ala 536 (Accession # P15423-1).
Predicted N-terminus: Cys 16

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 58.8 kDa. The protein migrates as 70-110 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Triterpene Derivatives as Potential Inhibitors of the RBD Spike Protein from SARS-CoV-2: An In Silico Approach"
Avelar, Pedraza-González, Sinicropi et al
Molecules (2023) 28 (5)
(2) "Folic Acid and Leucovorin Have Potential to Prevent SARS-CoV-2-Virus Internalization by Interacting with S-Glycoprotein/Neuropilin-1 Receptor Complex"
Škrbić, Travar, Stojiljković et al
Molecules (2023) 28 (5)
(3) "Comparative humoral response of mRNA and inactivated vaccines against COVID-19 in healthy adults aged 60 years and older"
Güvenir, Yürüker, Yetkin et al
J Infect Dev Ctries (2023) 17 (2), 178-181
Showing 1-3 of 2590 papers.