Recombinant SARS Nucleocapsid protein, His Tag, 100µg

Background
Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.

Source
Recombinant SARS Nucleocapsid protein, His Tag (NUN-S5229) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 422 (Accession # NP_828858.1).
Predicted N-terminus: Met 1

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.7 kDa. The protein migrates as 30-33 kDa and 55-60 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, Arginine, pH7.4, with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Liquid-liquid phase separation of nucleocapsid proteins during SARS-CoV-2 and HIV-1 replication"
Chau, Chen, Cochrane et al
Cell Rep (2022) 42 (1), 111968
(2) "Reactivity of NiII, PdII and PtII complexes bearing phosphine ligands towards ZnII displacement and hydrolysis in Cis2His2 and Cis3His zinc-fingers domains"
Galuppo, Gomes de Oliveira Junior, Dos Santos Oliveira et al
J Inorg Biochem (2022)
(3) "Proteomic screening identifies RPLp2 as a specific regulator for the translation of coronavirus"
Dong, Wang, Zhang et al
Int J Biol Macromol (2023) 230, 123191
Showing 1-3 of 53609 papers.