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Recombinant SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y), His Tag (MALS verified),100µg  

Recombinant SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y), His Tag (MALS verified),100µg

Recombinant SARS-CoV-2 (COVID-19) S protein RBD (K417N, E484K, N501Y), AA Arg 319 - Lys 537, expressed from human 293 cells (HEK293), (MALS verified), His Tag

Synonyms: Recombinant, Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD, COVID-19

More details

SPD-C52Hp-100

Availability: within 7 days

480,00 €

Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Source
Recombinant SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), His Tag (SPD-C52Hp) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (K417N, E484K, N501Y)). K417N / E484K / N501Y mutations were identified in the SARS-CoV-2 variant (known as 20C/501Y.V2 or B.1.351 lineage) which emerged in South Africa.Predicted N-terminus: Arg 319

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 26.6 kDa. The protein migrates as 33-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Bioactivity
Please refer to product data sheet.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.