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Recombinant Biotinylated Cynomolgus Fc gamma RIII / CD16 Protein, His,Avitag™ (MALS & BLI verified), 200 µg  

Recombinant Biotinylated Cynomolgus Fc gamma RIII / CD16 Protein, His,Avitag™ (MALS & BLI verified), 200 µg

Recombinant Biotinylated Cynomolgus Fc gamma RIII / CD16 Protein, AA Gly 17 - Gln 208, expressed from human 293 cells (HEK293), His,Avitag™ (MALS & BLI verified)

Synonyms: recombinant, cynomolgus, protein FCGR3, CD16

More details

FC6-C82E0-200

Availability: within 7 days

1 380,00 €

Background
CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody­dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

Source
Recombinant Biotinylated Cynomolgus CD16, His,Avitag (FC6-C82E0) is expressed from human 293 cells (HEK293). It contains AA Gly 17 - Gln 208 (Accession # Q8SPW2-1).
Predicted N-terminus: Gly 17

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 25.7 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Monocyte Subsets in Patients with Chronic Heart Failure Treated with Cardiac Resynchronization Therapy"
Ptaszyńska-Kopczyńska, Eljaszewicz, Marcinkiewicz-Siemion et al
Cells (2021) 10 (12)
(2) "[A comparative analysis of two methods for assessing the proinflammatory activity of monocytes in patients with juvenile depression]"
Vasilyeva, Sekirina, Omelchenko et al
Zh Nevrol Psikhiatr Im S S Korsakova (2021) 121 (11), 61-66
(3) "TIM3+ TRBV11-2 T cells and IFNγ signature in patrolling monocytes and CD16+ NK cells delineate MIS-C"
Hoste, Roels, Naesens et al
J Exp Med (2022) 219 (2)
Showing 1-3 of 2617 papers.

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