Recombinant Biotinylated Monkeypox virus (strain Zaire-96-I-16) E8L Protein, His,Avitag™ , 50µg

Background
Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The cases are mostly in central and western Africa. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa, multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions.

Source
Recombinant Biotinylated Monkeypox virus (strain Zaire-96-I-16) E8L Protein, His,Avitag (E8L-M82E3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 275 (Accession # Q8V4Y0-1).
Predicted N-terminus: Met 1.

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 35.4 kDa. The protein migrates as 44-54 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Biotinylation
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Biotin:Protein Ratio
Passed as determined by the HABA assay / binding ELISA.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "A Vaccine Strategy Based on the Identification of an Annular Ganglioside Binding Motif in Monkeypox Virus Protein E8L"
Fantini, Chahinian, Yahi
Viruses (2022) 14 (11)
(2) "Inhibition of monkeypox virus replication by RNA interference"
Alkhalil, Strand, Mucker et al
Virol J (2009) 6, 188
(3) "MFG-E8 in the retina and retinal pigment epithelium of rat and mouse"
Burgess, Abrams, Nagata et al
Mol Vis (2006) 12, 1437-47
Showing 1-3 of 4 papers.