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Recombinant Monkeypox virus (strain Zaire-96-I-16) A30L Protein, His Tag (MALS verified), 50µg  

Recombinant Monkeypox virus (strain Zaire-96-I-16) A30L Protein, His Tag (MALS verified), 50µg

Recombinant Monkeypox virus (strain Zaire-96-I-16) A30L Protein, AA Ser 22 - Leu 146, expressed from human 293 cells (HEK293), His Tag (MALS verified)

Synonyms: Recombinant, protein, A30L, monkey pox

More details

A3L-M5243-50

Availability: within 7 days

611,00 €

Background
Monkeypox is a rare zoonosis caused by monkeypox virus, which has become the most serious orthpoxvirus and consists of complex double stranded DNA. The cases are mostly in central and western Africa. The pathogenesis of monkeypox is that the virus invades the body from respiratory mucosa , multiplies in lymphocytes, and incurs into blood producing transient venereal toxemia. after the virus multiplies in cells, the cells can invade the blood and propagate to the skin of the whole body, causing lesions. A30L is a Envelope protein required for virus entry into host cell and for cell-cell fusion (syncytium formation).

Source
Recombinant Monkeypox virus (strain Zaire-96-I-16) A30L, His Tag (A3L-M5243) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Leu 146 (Accession # Q8V4U9-1).
Predicted N-terminus: His

Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 16.0 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Bioactivity
Please refer to product data sheet.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Immunoinformatic approach for rational identification of immunogenic peptides against host entry and/or exit monkeypox proteins and potential multi-epitope vaccine construction"
de Araújo, Santos, Corsetti et al
J Infect Dis (2023)
(2) "Computer-Aided Multi-Epitope Based Vaccine Design Against Monkeypox Virus Surface Protein A30L: An Immunoinformatics Approach"
Ramprasadh, Rajakumar, Srinivasan et al
Protein J (2023) 42 (6), 645-663
(3) "Mpox vaccine and infection-driven human immune signatures: an immunological analysis of an observational study
Cohn, Bloom, Cai et al
Lancet Infect Dis (2023) 23 (11), 1302-1312
Showing 1-3 of 20 papers.