Recombinant human Akt2 / PKB beta, active protein kinase 40 µg

Background: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a pleckstrin homology (PH) domain. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration. Human recombinant protein kinase PKB beta expressed in Sf9 cells.

Expression system: Sf9 cells
Purification: One-step affinity purification using Ni-NTA agarose
Purity: > 95% by SDS PAGE
Theoretical MW: 60.4 kDa
Storage buffer: 50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 50 % glycerol, pH 8.5.
Storage temperature: - 80°C (avoid repeated freeze-thaw cycles !)
Protein concentration: 1.54 mg/ml (Bradford with BSA as standard protein)
Specific activity: 6100 U*/mg (1 Unit is defined as 1 picomole phosphate transferred to synthetic peptide (RPRAATF) per min at 30 °C)

Entrez Gene ID: 208 
UniProtKB:  P31751

Ordering information: shipped on dry ice

Product specific literature references:

Walker KS, Deak M, Paterson A, Hudson K, Cohen P, Alessi DR (1998) "Activation of protein kinase B beta and gamma isoforms by insulin in vivo and by 3-phosphoinositide-dependent protein kinase-1 in vitro: comparison with protein kinase B alpha" Biochem. J. 331 ( Pt 1):299-308

Okano J, Gaslightwala I, Birnbaum MJ, Rustgi AK, Nakagawa H (2000) "Akt/protein kinase B isoforms are differentially regulated by epidermal growth factor stimulation" J. Biol. Chem. 275(40):30934-42

Cho H, Mu J, Kim JK, Thorvaldsen JL, Chu Q, Crenshaw EB 3rd, Kaestner KH, Bartolomei MS, Shulman GI, Birnbaum MJ (2001) "Insulin resistance and a diabetes mellitus-like syndrome in mice lacking the protein kinase Akt2 (PKB beta)" Science 292(5522):1728-31

Vandromme M, Rochat A, Meier R, Carnac G, Besser D, Hemmings BA, Fernandez A, Lamb NJ (2001) "Protein kinase B beta/Akt2 plays a specific role in muscle differentiation" J. Biol. Chem. 276(11):8173-9

Garofalo RS, Orena SJ, Rafidi K, Torchia AJ, Stock JL, Hildebrandt AL, Coskran T, Black SC, Brees DJ, Wicks JR, McNeish JD, Coleman KG (2003) "Severe diabetes, age-dependent loss of adipose tissue, and mild growth deficiency in mice lacking Akt2/PKB beta" J. Clin. Invest. 112(2):197-208

Bae SS, Cho H, Mu J, Birnbaum MJ (2003) "Isoform-specific regulation of insulin-dependent glucose uptake by Akt/protein kinase B" J. Biol. Chem. 278(49):49530-6