Recombinant human Akt1 / PKB alpha (aa1-480), active protein kinase, 10 µg

Background: Protein kinase B or Akt (PKB/Akt) is a serine/threonine kinase, which in mammals comprises three highly homologous members known as PKB alpha (Akt1), PKB beta (Akt2) and PKB gamma (Akt3). PKB/Akt is a growth-factor-regulated protein kinase which contains a pleckstrin homology (PH) domain. Binding of phosphoinositide 3-OH kinase products to the pleckstrin homology domain results in translocation of PKB/Akt to the plasma membrane where it is activated by phosphorylation by upstream kinases including the phosphoinoside-dependent kinase 1 (PDK1). Key roles for this enzyme can be found in cellular processes such as glucose metabolism, cell proliferation, apoptosis, transcription and cell migration. Human recombinant and active enzyme PKB alpha expressed in Sf9 cells.

Theoretical MW: 81.2 kDa (fusion protein)
Expression system: Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol
Protein concentration: 0.22 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MSFC membrane
Specific activity: 55.000 pmol/mg x min

Entrez Gene ID: 207 
UniProtKB: P31749

Ordering information: shipped on dry ice

BIAFFIN – SERVICES:

Use the PKB / Akt - SPR Binding Assay of BIAFFIN for comprehensive kinetic characterization of your small molecule kinase inhibitors. 

Figure: Real-time kinetic analysis of kinase inhibitor MK-2206 binding to PKBα / Akt1 using surface plasmon resonance.

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Product specific literature references:

• Downward J (1998) "Mechanisms and consequences of activation of protein kinase B/Akt" Curr Opin Cell Biol. 10(2):262-7

• Brazil DP, Hemmings BA (2001) "Ten years of protein kinase B signalling: a hard Akt to follow" Trends Biochem Sci. 26(11):657-64

• Nicholson KM, Anderson NG (2002) "The protein kinase B/Akt signalling pathway in human malignancy" Cell Signal. 14(5):381-95