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Recombinant human checkpoint homologue kinase (CHK2), active protein kinase, 20 µg  

Recombinant human checkpoint homologue kinase (CHK2), active protein kinase, 20 µg

Recombinant human checkpoint homologue kinase (CHK2), active enzyme

Alternate names: CDS1, HuCds1, LFS2, PP1425, RAD53, CHEK2, checkpoint homolog, CDS1, CHK2

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PK-CHK2-A020

Availability: within 3 days

295,00 €

Checkpoint kinase 2 (CHK2) is multifunctional player in the DNA-damage response signalling pathway, especially at the S and G2 checkpoints of cell cycle. The protein consits of three functional domains. The T-terminal SQ/TQ cluster (SCD motif), the forkhead associated domain (FHA) and the kinase domain. Following DNA damage, ATM or ATR kinase phosphorylates CHK2 at the SCD motif.  Thereafter dimerization and autophosphorylation takes place. Activated CHK2 phosphorylates e.g. Cdc25A, B, and C and E2F-1 and causes cell cycle arrest and apoptosis.

Affinity-purified recombinant human checkpoint homologue kinase (CHK2) expressed in insect cells featuring an affinity tag. 

Highly active form of CHK2 suitable for labeling CHK substrates

Protein concentration (Bradford with BSA as standard)         0.4 mg/ml
Purity                                        > 95% by SDS PAGE
Specific activity                        > 142,600 U*/mg

Molecular weight based on amino acid sequence 66 kDa

Entrez Gene ID: 11200 
UniProtKB: O96017

Ordering information: shipped on dry ice

Product specific literature references:

Matsuoka S, Huang M, Elledge SJ (1998) "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase" Science 282(5395):1893-7

Chaturvedi P, Eng WK, Zhou BB et al. (1999) "Mammalian Chk2 is a downstream effector of the ATM-dependent DNA damage checkpoint pathway" Oncogene 18(28):4047-54

Matsuoka S, Rotman G, Ogawa A, Shiloh Y, Tamai K, Elledge SJ (2000) "Ataxia telangiectasia-mutated phosphorylates Chk2 in vivo and in vitro" Proc. Natl. Acad. Sci. U S A 97(19):10389-94

Melchionna R, Chen XB, Blasina A, McGowan CH (2000) "Threonine 68 is required for radiation-induced phosphorylation and activation of Cds1" Nat. Cell. Biol. 2(10):762-5

Chehab NH, Malikzay A, Appel M, Halazonetis TD (2000) "Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53" Genes Dev. 14(3):278-88

Falck J, Mailand N, Syljuasen RG, Bartek J, Lukas J (2001) "The ATM-Chk2-Cdc25A checkpoint pathway guards against radioresistant DNA synthesis" Nature 410(6830):842-7

Bartek J, Falck J, Lukas J (2001) "CHK2 kinase--a busy messenger" Nat. Rev. Mol. Cell. Biol. 2(12):877-86

McGowan CH (2002) "Checking in on Cds1 (Chk2): A checkpoint kinase and tumor suppressor" Bioessays 24(6):502-11