Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant human CTLA4/CD152 Protein, His Tag, 100µg  

Recombinant human CTLA4/CD152 Protein, His Tag, 100µg

Recombinant Human CTLA4 /CD152 Protein (rhCTLA4) Ala 37 - Phe 162, produced in human 293 cells (HEK293), His Tag

Synonym
recombinant human protein CTLA4, CD152

More details

CT4-H5229-100

Availability: within 7 days

154,00 €

Background
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. T cell activation through the T cell receptor and CD28 leads to increased expression of CTLA-4, an inhibitory receptor for B7 molecules. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

Source
Recombinant Human CTLA4 /CD152 Protein (rhCTLA4) Ala 37 - Phe 162 (Accession # NP_005205.2) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.4 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to different extent glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhCTLA4 by the LAL method.

Purity
>95% purity as determined by SDS-PAGE.

Formulation
Refer to data sheet

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 1 month (4°C-8°C) or 3 months (-20°C to -70°C).

Bioactivity
Immobilized Human B7-2, Fc Tag (Catalog # CD6-H5257) at 2μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Catalog # CT4-H5229) with a linear range of 1-6.4 ng/mL.
Immobilized Human B7-1, Fc Tag (Catalog # B71-H5259) at 2μg/mL (100 μL/well) can bind Human CTLA-4, His Tag (Catalog # CT4-H5229) with a linear range of 0.16-2.56 ng/mL.

References

(1) Waterhouse P, et al., 1995, Science 270 (5238): 985–8.
(2) Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596–602.
(3) Rudd, CE. et al., 2009, Immunol. Rev. 229 (1): 12-26.