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Recombinant Cynomolgus / Rhesus macaque CTLA-4 Protein, His Tag, 200µg  

Recombinant Cynomolgus / Rhesus macaque CTLA-4 Protein, His Tag, 200µg

Recombinant Cynomolgus / Rhesus macaque CTLA4 / CD152 Protein, Ala 37 - Ser 160, produced in human 293 cells (HEK293), His Tag

Synonym
Recombinant Cynomolgus Protein, CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12

More details

CT4-C5227-200

Availability: within 7 days

216,00 €

Background: CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

Source
Recombinant Cynomolgus / Rhesus macaque CTLA-4, His Tag (CT4-C5227) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Ser 160 (Accession # G7PL88).

Molecular Characterization
Cynomolgus CTLA4 /CD152, fused with 6×His tag at the C-terminus, has a calculated MW of 14.1 kDa. The predicted N-terminus is Ala 37. DTT-reduced Protein migrates as 18-23 kDa due to glycosylation

Endotoxin
Less than 1.0 EU per μg of the Cynomolgus CTLA4 /CD152 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

Bioactivity
Immobilized Cynomolgus / Rhesus macaque B7-1, Fc Tag (Cat. No. CD0-C5252) at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CTLA-4, His Tag (Cat. No. CT4-C5227) with a linear range of 0.2-2 ng/mL (QC tested).
Immobilized Cynomolgus / Rhesus macaque B7-2, Fc Tag (Cat. No. CD6-C5254) at 2 μg/mL (100 μL/well) can bind Cynomolgus / Rhesus macaque CTLA-4, His Tag (Cat. No. CT4-C5227) with a linear range of 4-31 ng/mL (Routinely tested).

References

(1) Waterhouse P, et al., 1995, Science 270 (5238): 985–8.
(2) Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596–602.
(3) Rudd, CE. et al., 2009, Immunol. Rev. 229 (1): 12-26.

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