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Recombinant Human CTLA4 /CD152 Protein, With C-Fc Tag, 200µg  

Recombinant Human CTLA4 /CD152 Protein, With C-Fc Tag, 200µg

Recombinant Human CTLA4 /CD152 Protein, With C-Fc Tag (rh CTLA4 /CD152 Fc Chimera) Ala 37 - Ser 160 was produced in human HEK293 cells

Synonym
recombinant human protein CTLA4, CD152, CELIAC3, GRD4, GSE, ICOS, IDDM12

More details

CT4-H5255-200

Availability: within 7 days

216,00 €

Background
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

Source
Recombinant Human CTLA4 /CD152 Protein, With C-Fc Tag (rh CTLA4 /CD152 Fc Chimera) Ala 37 - Ser 160 (Accession # NP_005205.2) was produced in human 293 cells (HEK293).

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 40.0 kDa. As a result of glycosylation, the protein migrates as 45-55 kDa under reducing (R) condition, and 90 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg of the rh CTLA4 /CD152 Fc Chimera by the LAL method.

Purity
>98% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

Bioactivity
Please refer to product data sheet.

References

(1) Waterhouse P, et al., 1995, Science 270 (5238): 985–8.
(2) Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596–602.
(3) Rudd, CE. et al., 2009, Immunol. Rev. 229 (1): 12-26.