Recombinant Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag, 100 µg

Background
T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.

Source
Recombinant Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (MALS verified) (CDD-M5219) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P22646-1(CD3E) & P04235-1(CD3D)).
Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)

Molecular Characterization
Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.1 kDa (CD3E) and 14.3 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D is fused with a flag tag at the C-terminus. The reducing (R) heterodimer protein migrates as 19 kDa and 25-33 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Single-Cell RNA and ATAC Sequencing Reveal Hemodialysis-Related Immune Dysregulation of Circulating Immune Cell Subpopulations"
Wu, Dong, Yu et al
Front Immunol (2022) 13, 878226
(2) "Severe combined immunodeficiencies: Expanding the mutation spectrum in Turkey and identification of 12 novel variants"
Aykut, Durmaz, Karaca et al
Scand J Immunol (2022) 95 (6), e13163
(3) "Transcriptome-Based Molecular Networks Uncovered Interplay Between Druggable Genes of CD8+ T Cells and Changes in Immune Cell Landscape in Patients With Pulmonary Tuberculosis"
Alsulaimany, Zabermawi, Almukadi et al
Front Med (Lausanne) (2021) 8, 812857
Showing 1-3 of 37 papers.