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Recombinant Rat ROR1 Protein, full ECD, His tag, 100 µg  

Recombinant Rat ROR1 Protein, full ECD, His tag, 100 µg

Recombinant Rat ROR1 Protein, full extracellular domain (Gln 30 - Glu 403), expressed from human 293 cells (HEK293), His tag

Synonym: recombinant rat protein, ROR1, NTRKR1

More details

RO1-R5221-100

Availability: within 7 days

420,00 €

Background
Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm. ROR1 could interact with casein kinase 1 epsilon (CK1ε) to activate phosphoinositide 3-kinase-mediated AKT phosphorylation and cAMP-response-element-binding protein (CREB), which was associated with enhanced tumor-cell growth.

Source
Recombinant Rat ROR1 protein, His Tag (RO1-R5221) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Glu 403 (Accession # EDL97819).
Predicted N-terminus: Gln 30

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 43.5 kDa. The protein migrates as 60-67 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

References

(1) Masiakowski P., et al., 1992, J. Biol. Chem. 267:26181-26190.
(2) Reddy U.R., et al., 1996, Oncogene 13:1555-1559.
(3) Greenman C., et al., 2007, Nature 446:153-158.