Recombinant Human Glypican 2 / GPC2 Protein, Fc Tag, 100 µg

Background
Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.

Source
Recombinant Human Glypican 2, Fc Tag (GP2-H5255) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Ser 553 (Accession # Q8N158-1).

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 84.6 kDa. The protein migrates as 120k Da under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "CpG methylation changes in human mesenchymal and neural stem cells in response to in vitro niche modifications"
Gyimesi, Oikari, Yu et al
Biochimie (2024)
(2) "Synthesis and preclinical evaluation of a novel probe [18F]AlF-NOTA-IPB-GPC3P for PET imaging of GPC3 positive tumor"
Mo, Sun, Liang et al
Bioorg Chem (2024) 147, 107352
(3) "Dichotomous Effects of Glypican-4 on Cancer Progression and Its Crosstalk with Oncogenes"
Chérouvrier Hansson, Cheng, Georgolopoulos et al
Int J Mol Sci (2024) 25 (7)
Showing 1-3 of 1487 papers.