Recombinant Human Glypican 2 / GPC2 Protein, His Tag, 100 µg

Background
Glypican 2 (GPC2), also known cerebroglycan, is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1. GPC2 silencing inactivates Wnt/β-catenin signaling and reduces the expression of N-Myc, an oncogenic driver of neuroblastoma tumorigenesis. Immunotoxins and chimeric antigen receptor (CAR) T cells targeting GPC2 inhibit neuroblastoma growth in mouse models. A GPC3 specific antibody drug conjugate (ADC) can also inhibit neuroblastoma cell proliferation.

Source
Recombinant Human Glypican 2, His Tag (GP2-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Gly 554 (Accession # Q8N158-1).
Predicted N-terminus: Ser 24

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 60.0 kDa. The protein migrates as 55-120 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Bioactivity
Please refer to product data sheet.

Research Progress of Glypican 2

(1) "Investigation of Glypican-4 and -6 by Infrared Spectral Imaging during the Hair Growth Cycle"
Colin-Pierre, Untereiner, Sockalingum et al
Int J Mol Sci (2023) 24 (5)
(2) "Differential lncRNA/mRNA expression profiling and ceRNA network analyses in amniotic fluid from foetuses with ventricular septal defects"
Wang, Lin, Wang et al
PeerJ (2023) 11, e14962
(3) "Glypican-3, Vascular Endothelial Growth Factor and Golgi Protein-73 for Differentiation between Liver Cirrhosis and Hepatocellular Carcinoma"
Mamdouh, Soliman, Khorshed et al
Asian Pac J Cancer Prev (2023) 24 (2), 497-507
Showing 1-3 of 1387 papers.