Recombinant Mouse Glypican 3 / GPC3 Protein, His Tag, 100 µg

Background
Glypican-3 (GPC3) is also known as Intestinal protein OCI-5, GTR2-2, MXR7, which belongs to the glypican family. Glypican 3 / GPC-3 is highly expressed in lung, liver and kidney. Glypican-3 inhibits the dipeptidyl peptidase activity of DPP4. Glypican-3 may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and also may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function.

Source
Recombinant Mouse Glypican 3 / GPC3 protein, His Tag (GP3-M52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Met 557 (Accession # Q8CFZ4-1).
Predicted N-terminus: Gln 25 & Ser 358

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin-like convertase cleavage site, 354-RQYR-357, and will be partially processed into N and C-terminal fragment with calculated MW of 38.0 kDa and 24.4 kDa respectively. The protein migrates as 38-40 kDa and 62-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.
 
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.
 
Bioactivity
Please refer to product data sheet.

Clinical and Translational Updates

(1) "Hematogenous Dissemination of Tumor Cells in Hepatocellular Carcinoma: Comparing Anterior and Non-anterior Approach Hepatectomy"
Sasaki, Nomura, Sudo et al
Anticancer Res (2022) 42 (8), 4129-4137
(2) "Nonviral mcDNA-mediated bispecific CAR T cells kill tumor cells in an experimental mouse model of hepatocellular carcinoma"
Wang, Wang, Ye et al
BMC Cancer (2022) 22 (1), 814
(3) "Determination of starting dose of the T cell-redirecting bispecific antibody ERY974 targeting glypican-3 in first-in-human clinical trial"
Komatsu, Kayukawa, Miyazaki et al
Sci Rep (2022) 12 (1), 12312
Showing 1-3 of 1767 papers.