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ActiveMax® Recombinant Human IL-4 Protein, 20µg  

ActiveMax® Recombinant Human IL-4 Protein, 20µg

ActiveMax® Recombinant Human IL-4, His 25 - Ser 153, produced in human 293 cells (HEK293), Tag free

Synonym: Recombinant Human Protein, IL4,BCGF1,BSF1

More details

IL4-H4218-20

Availability: within 7 days

216,00 €

Background
Interleukin-4, is a cytokine that induces differentiation of naive helper T cells (Th0 cells to Th2 cells). In the presence of IL-4 and IL-13, cytokines that are produced in a Th-2 type response, particularly during allergy and parasitic infections, macrophages become differentially activated, And this cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. Recently, researcher found that the cytokine IL-4 plays a key role in development of innate CD8+ T cells in the thymus of several gene-deficient mouse strains, including Itk, KLF2, CBP and Id3, without previous exposure to antigen.

Source
ActiveMax® Recombinant Human IL-4 Protein, His 25 - Ser 153 (Accession # AAH67514) was produced in human 293 cells (HEK293)

Molecular Characterization
ActiveMax® Human IL-4 contains no "tag", and has a calculated MW of 14.9 kDa. The predicted N-terminus is His 25. The reducing (R) protein migrates as 15 and 19 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 0.1 EU per μg by the LAL method.

Purity
>90% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS and NaCl. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human IL-4, Tag Free (Catalog # IL4-H4218) at 5μg/mL (100 μL/well) can bind Human IL-4 R alpha, His Tag (Catalog # ILR-H5221) with a linear range of 0.01-0.1 μg/mL. The bio-activity was determined by dose-dependent stimulation of the proliferation of TF-1 cells. The ED50 < 0.2 ng/mL, corresponding to a specific activity of >5000000 Unit/mg. 

References

(1) Callard RE., 1991, Br J Haematol, 78(3): 293-9.
(2) Varin A, Gordon S., et al., 2009, Immunobiology. 214(7):630-41.
(3) Finkelman FD, et al., 2004, Immunol Rev. 201:139-55.
(4) Lee YJ, et al., 2011, Trends Immunol, 32(2):50-6.