Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Cynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, Fc Tag, 100µg  

Cynomolgus / Rhesus macaque PD-L1 / B7-H1 Protein, Fc Tag, 100µg

Recombinant Cynomolgus / Rhesus macaque PDL1 /CD274 Protein, C-Fc Tag (Cynomolgus / Rhesus macaque PDL1 /CD274 Fc Chimera) Phe 19 - Arg 238 was produced in human 293 cells (HEK293)

Synonym
recombinant cynomolgus protein PD-L1, CD274, B7-H1, PDCD1L1, PDCD1LG1

More details

PD1-C5253-100

Availability: within 7 days

300,00 €

Background
Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells. Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance. PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.

Source
Recombinant Cynomolgus / Rhesus macaque PD-L1, Fc Tag (PD1-C5253) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # G7PSE7).

Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus PD-1, His Tag (Catalog # PD1-C5223) at 10 μg/mL (100 μL/well) can bind Cynomolgus PD-L1, Fc Tag (Catalog # PD1-C5253) with a linear range of 0.156-5 μg/mL.

Molecular Characterization
Cynomolgus PDL1 /CD274 Fc Chimera is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 51.8 kDa. The predicted N-terminus is Phe 19. DTT-reduced Protein migrates as 66-80 kDa in SDS-PAGE due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the Cynomolgus PDL1 /CD274 Fc Chimera by the LAL method.

Purity
>90% as determined by SEC-HPLC and SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C-8°C); After reconstitution under sterile conditions for 3 months (-20°C to -70°C).

References

(1) Dong H., et al., 1999, Nat. Med. 5:1365-1369.
(2) Freeman G.J., et al., 2000, J. Exp. Med. 192:1027-1034.
(3) Nishimura H., et al., 2001, Trends in Immunology 22:265.