Recombinant Mouse IL-13 R alpha 1 Protein, His Tag, 100µg

Background
Interleukin 13 receptor, alpha 1 is also known as IL13RA1, NR4 and CD213A1 (cluster of differentiation 213A1), The IL13 Rα1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouseIL13Rα1 share 76% aa sequence identity. IL13RA1 is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4.

Source
Recombinant Mouse IL-13 R alpha 1, His Tag (IL1-M52H7) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Thr 340 (Accession # NP_598751).
Predicted N-terminus: Ala 26

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 37.9 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose is added as protectant before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Etanercept alleviates psoriasis by reducing the Th17/Treg ratio and promoting M2 polarization of macrophages"
Li, Jiang, Chen et al
Immun Inflamm Dis (2022) 10 (12), e734
(2) "Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function"
Liao, Foo, Tran et al
Br J Pharmacol (2022)
(3) "The impact of the PCSK-9/VLDL-Receptor axis on inflammatory cell polarization"
Barcena, Estepa, Marx et al
Cytokine (2023) 161, 156077
Showing 1-3 of 193 papers.