Products

Biaffin

Newsletter

kinases protein kinase inhibitors signaling activators kinase substrates antibodies bioluminescence assays
Recombinant Human Nectin-1 / PVRL1 / CD111 Protein, His tag, 100µg  

Recombinant Human Nectin-1 / PVRL1 / CD111 Protein, His tag, 100µg

Recombinant Human PVRL1 /Nectin-1 /CD111 Protein (Gln 31 - Thr 334), produced in human 293 cells (HEK293), His tag

Synonym
Recombinant human protein, PVRL1, Nectin-1, CD111, CLPED1, ED4, HIgR, HVEC, OFC7, PRR, PRR1, PVRR, PVRR1, SK-12

More details

PV1-H5223-100

Availability: within 7 days

300,00 €

Background
Poliovirus receptor-related protein 1 (PVRL1) is also known as Herpes virus entry mediator C (HveC), Herpesvirus Ig-like receptor (HIgR), Nectin-1, CD antigen CD111, PVRL1 / Nectin-1 / CD111 belongs to the nectin family. PVRL1 / Nectin-1 is a membrane protein with three extracellular immunoglobulin domains, a single transmembrane helix and a cytoplasmic tail. The protein can mediate Ca2+-independent cellular adhesion further characterizing it as IgSF cell adhesion molecule (IgSF CAM). PVRL1 is adhesion molecule found in a wide range of tissues. PVRL1 / Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between PVRL1/nectin-1 and PVRL3/nectin-3 and between PVRL1/nectin-1 and PVRL4/nectin-4.

Source
Recombinant Human PVRL1 /Nectin-1 /CD111 Protein (rhPVRL1 /Nectin-1) Gln 31 - Thr 334 (Accession # NP_002846) was produced in human 293 cells (HEK293) at ACRObiosystems.

Molecular Characterization
rhPVRL1 /Nectin-1, fused with 6×His tag at the C-terminus, has a calculated MW of 34.8 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 45-60 kDa due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rhPVRL1 /Nectin-1 by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Bioactivity-ELISA
Human Nectin-1, His TagHuman Nectin-1, His Tag (Cat. No. PV1-H5223) ELISA bioactivity
Immobilized Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) at 2 μg/mL (100 μL/well) can bind Human Nectin-1, His Tag (Cat. No. PV1-H5223) with a linear range of 0.078-1.25 μg/mL (QC tested).

References

(1) Lopez M., et al., 1995, Gene 155:261-265.
(2) Suzuki K., et al., 2000, Nat. Genet. 25:427-430.