Recombinant Cynomolgus / Rhesus macaque beta 2-Microglobulin / B2M Protein, 100 µg

Background
β2 microglobulin is also known as Beta-2-microglobulin (B2M), is a component of MHC class I molecules which belongs to the beta-2-microglobulin family. B2M is present on all nucleated cells (excludes red blood cells). B2M associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 and Qa. An additional function of B2M is association with the HFE protein, together regulating the expression of hepcidin in the liver which targets the iron transporter ferroportin on the cytoplasmic membrane of enterocytes and macrophages for degradation resulting in decreased iron uptake from food and iron release from recycled red blood cells respectively. Loss of this function causes iron excess and hemochromatosis. Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP).

Source
Recombinant Cynomolgus / Rhesus macaque B2M, His Tag (B2M-C5226) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Met 119 (Accession # Q8SPW0). In the region Ile 21 - Met 119, the AA sequence of Cynomolgus and Rhesus macaque beta 2-Microglobulin are homologus.
Predicted N-terminus: Ile 21

Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 12.6 kDa. The protein migrates as 14 kDa under reducing (R) condition (SDS-PAGE).

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Hemodiafiltration with endogenous reinfusion for uremic toxin removal in patients undergoing maintenance hemodialysis: a pilot study"
Lu, Fang, Wu et al
Ren Fail (2024) 46 (1), 2338929
(2) "A study of size-selective renal elimination using a novel human model"
Sigurjonsson, Grubb, Grubb et al
Scand J Clin Lab Invest (2024)
(3) "Acute Kidney Injury Prediction Model Using Cystatin-C, Beta-2 Microglobulin, and Neutrophil Gelatinase-Associated Lipocalin Biomarker in Sepsis Patients"
Susianti, Asmoro, Sujarwoto et al
Int J Nephrol Renovasc Dis (2024) 17, 105-112
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