Recombinant Human Frizzled-7 / FZD7 Protein, Fc Tag, 100 µg

Background
Frizzled-7 (FZD7) is also known as FzE3, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD7 contains one FZ (frizzled) domain. FZD7 is receptor for Wnt proteins. FZD7 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. FZD7 interacts with MAGI3 and DVL1.

Source
Recombinant Human FZD7 /Frizzled-7 Protein, Fc Tag (FZ7-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 33 - Leu 185 (Accession # AAH15915).
Predicted N-terminus: Gln 33

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 43.3 kDa. The protein migrates as 50-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "USP30-AS1 Dictates Breast Cancer Cell Fate and Chemoresistance via a miR-3646/FZD7/Wnt/β-Catenin Circuit"
He, Yang, Xin et al
Curr Issues Mol Biol (2025) 47 (12)
(2) "In silico docking yields small molecule negative allosteric modulators targeting the core of Frizzled 7"
Scharf, Kinsolving, Grätz et al
Nat Commun (2025) 16 (1), 11138
(3) "N6-methyladenosine regulated FZD7 inhibits ferroptosis in endometriosis via β-catenin/SLC7A11 pathway"
Huang, Shi, Liao et al
Free Radic Biol Med (2026) 243, 54-66
Showing 1-3 of 270 papers.