Recombinant Human Frizzled-7 / FZD7 Protein, Fc Tag, 100 µg

Background
Frizzled-7 (FZD7) is also known as FzE3, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD7 contains one FZ (frizzled) domain. FZD7 is receptor for Wnt proteins. FZD7 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. FZD7 interacts with MAGI3 and DVL1.

Source
Recombinant Human FZD7 /Frizzled-7 Protein, Fc Tag (FZ7-H5258) is expressed from human 293 cells (HEK293). It contains AA Gln 33 - Leu 185 (Accession # AAH15915).
Predicted N-terminus: Gln 33

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 43.3 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70^°C for 3 months under sterile conditions after reconstitution.

Clinical and Translational Updates

(1) "Frizzled-7 modulates Goblet and Paneth cell fate and maintains the homeostasis in intestines"
Gu, Guo, Lin et al
Development (2023)
(2) "Frizzled-7-targeting antibody (SHH002-hu1) potently suppresses non-small-cell lung cancer via Wnt/β-catenin signaling"
Li, Mao, Li et al
Cancer Sci (2023)
(3) "MiR-1-3p enhances the sensitivity of ovarian cancer cells to ferroptosis by targeting FZD7"
Zhang, Qu, Hu et al
Zhong Nan Da Xue Xue Bao Yi Xue Ban (2022) 47 (11), 1512-1521
Showing 1-3 of 157 papers.