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Recombinant Human Frizzled-5 / FZD5 Protein, Fc Tag, 100 µg  

Recombinant Human Frizzled-5 / FZD5 Protein, Fc Tag, 100 µg

Recombinant Human FZD5 /Frizzled-5 Protein (Ala 27 - Pro 167), produced in human 293 cells (HEK293), Fc Tag

Synonym
Recombinant human protein, FZD5, Frizzled-5, C2orf31, , Fz-5, hFz5, FzE5

More details

FZ5-H5259-100

Availability: within 7 days

312,00 €

Background
Frizzled-5 (FZD5) is also known as FzE5, which belongs to the G-protein coupled receptor Fz/Smo family. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. FZD5 contains one FZ (frizzled) domain. FZD5 may be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. FZD5 interacts specifically with Wnt5A to induce the beta-catenin pathway. FZD5 interacts with GOPC.

Source
Recombinant Human FZD5 /Frizzled-5 Protein, with C-Fc Tag (rh FZD5 / Frizzled-5 Fc Chimera) Ala 27 - Pro 167 (Accession # Q13467) was produced in human 293 cells (HEK293) at ACROBiosystems.

Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 42.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin
Less than 1.0 EU per μg of the rh FZD5 / Frizzled-5 Fc Chimera by the LAL method.

Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution
See Certificate of Analysis for reconstitution instructions and specific concentrations.

Storage
Avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).
 

References

(1) Hsieh J.C., et al., 1999, Proc. Natl. Acad. Sci. U.S.A. 96:3546-3551.
(2) Hao H.X., et al., 2012, Nature 485:195-200.